Frequently Asked Questions

FAQ – GENERAL

When should I contact Utrecht Sequencing Facility?

If you need more information about NGS technology, DNA/RNA isolation, library preparation, SNP fingerprinting, bioinformatics data analysis and storage. And/or if you want to discuss your experiment/project that uses NGS technology

 

How should I deliver my samples?

For DNA / RNA isolation, SNP fingerprinting, library preparation: please refer to the services page. Additionally you need to submit your samples electronically. For ready-to-sequence samples: please deliver your (pooled) samples in an 1,5 ml tube at a concentration of 4 nM with a minimal volume of 40 ul. Additionally you need to submit your samples electronically. Hand over your samples to a USF employee in a 1.5 ml tube with the run ID written on the lid and the sample name on the lid/side.

 

When will my samples be ready?

Samples will be put in queue when online sample submission has been performed and samples have been physically handed over to the facility. We hold on to “first in, first sequenced” principle. Turn around times are two weeks for RNA / DNA isolations, two weeks for SNP fingerprinting, three weeks for library preparation and two weeks for sequencing run requests. Be aware that turn around times depend on the number of requests, available reagents and personnel. Furthermore, we cannot control machine outage. In case of significant delays, we will contact you. We are open for high priority requests. Note that we will discuss your request internally before granting.

 

How can I acknowledge Utrecht Sequencing Facility?

Utrecht Sequencing Facility is a joint initiative that is financially supported by the University Medical Center Utrecht, the Hubrecht Institute and the Utrecht University. To guarantee our continued existence, it is important to acknowledge the contribution of the Utrecht Sequencing Facility wherever possible. Please use the following standard text “We thank Utrecht Sequencing Facility for providing sequencing service and data. Utrecht Sequencing Facility is subsidized by the University Medical Center Utrecht, Hubrecht Institute and Utrecht University” 

 

Feedback and/or complaint?

We are committed to providing great service and support. Your feedback, whether it is a compliment, suggestion or complaint, is very valuable to us. Please contact us!

 

FAQ – SEQUENCING

What is paired-end sequencing?

Paired-end sequencing allows users to sequence both ends of a fragment and generate high-quality, alignable sequence data. Paired-end sequencing facilitates detection of genomic rearrangements and repetitive sequence elements, as well as gene fusions and novel transcripts.

Source: Illumina

paired-end-vs-single-read-seq-web-graphic