We facilitate the following library preparation methods using standard Illumina reagents, both as manual and automated (Neoprep) workflow:
- TruSeq DNA Nano LT (manual)
- TruSeq DNA Nano (Neoprep, automated)
- TruSeq Stranded total RNA, ribo zero for human/mouse/rat (manual)
- TruSeq Stranded mRNA polyA (manual)
- TruSeq Stranded mRNA polyA (Neoprep, automated)
A maximum of 24 TruSeq indexes is available. Please contact us if you would like to use custom indexes. Bioinformatics demultiplexing service is supported for a restricted set of indexes.
For DNA library preparation we request a minimum of 200 ng in 50 ul, measured with a fluorometric quantitation analysis (e.g. Qubit).
For RNA library preparation polyA-based (both Neoprep and manual) we request a minimum of 200 ng in 12.5 ul.
For RNA library preparation ribo zero (manual) we request a minimum of 500 ng in 15 ul.
RNA concentration should be measured with a fluorometric quantitation analysis (e.g. Qubit) and RNA quality should be at least RIN 8 (RNA Integrity Number).
Requester is responsible for collection of primary sample. Note that samples will be stored for a maximum of 2 months and subsequently discarded without further notice.
Service fees include costs for chemicals, operator time, quality control (assessed with Bioanalyzer 2100 and Qubit) and troubleshooting.
Library quality is mainly influenced by sample input quality. Users are responsible for ensuring that the quality of primary samples is high enough for successful library preparation. We will contact users when we have any doubt about input or library quality. Reagents that have been used will be charged, even if samples cannot be prepped and/or sequenced.