Frequently Asked Questions

FAQ – GENERAL

When should I contact Utrecht Sequencing Facility?

If you need more information about NGS technology, DNA/RNA isolation, library preparation, SNP fingerprinting, bioinformatics data analysis and storage. And/or if you want to discuss your experiment/project that uses NGS technology.

 

How should I deliver my samples?

Hand over your samples in a 1.5 ml tube with the run ID written on the lid and the sample name on the lid/side. You can put your samples in the “USEQ freezer” located next to the printer and the coffee corner, 2nd floor of the Stratenum. Additionally, you need to submit your samples electronically at the submission page.

For ready-to-sequence samples: please deliver your (pooled) samples in an 1,5 ml tube with a concentration of 4 nM and a minimal volume of 40 ul. For DNA / RNA isolation, SNP fingerprinting, library preparation: please refer to the services page for the specific delivery requirements per requested service.

In case of delicate samples such as RNA or unisolated cell pellets, please send a notification to useq@umcutrecht.nl after sample delivery at the USEQ freezer to make sure that your sample will be stored properly as soon as possible. We cannot take responsibility for decreased sample quality caused by prolonged and/or inappropriate storage.

 

When will my samples be ready?

Samples will be put in queue when online sample submission has been performed and samples have been physically handed over to the facility. We hold on to the “first in, first sequenced” principle. Turn around times are two weeks for RNA / DNA isolations, two weeks for SNP fingerprinting, three weeks for library preparation and two weeks for sequencing run requests. Be aware that turn around times depend on the number of requests, available reagents and personnel. Furthermore, we cannot control machine outage. In case of significant delays, we will contact you.

We are open for high priority requests. Note that we will discuss your request internally before granting.

 

How can I acknowledge Utrecht Sequencing Facility?

Utrecht Sequencing Facility is a joint initiative that is financially supported by the University Medical Center Utrecht, the Hubrecht Institute and the Utrecht University. To guarantee our continued existence, it is important to acknowledge the contribution of the Utrecht Sequencing Facility wherever possible. Please use the following standard text “We thank Utrecht Sequencing Facility for providing sequencing service and data. Utrecht Sequencing Facility is subsidized by the University Medical Center Utrecht, Hubrecht Institute and Utrecht University” 

 

Feedback and/or complaint?

We are committed to providing great service and support. Your feedback, whether it is a compliment, suggestion or complaint, is very valuable to us. Please contact us!

 

FAQ – SEQUENCING

What is paired-end sequencing?

Paired-end sequencing allows users to sequence both ends of a fragment and generate high-quality, alignable sequence data. Paired-end sequencing facilitates detection of genomic rearrangements and repetitive sequence elements, as well as gene fusions and novel transcripts.

Source: Illumina

paired-end-vs-single-read-seq-web-graphic